Figure 5.

CircPUM1 depletion causes HTR-8/SVneo cell dysfunction and inflammation via JUNB. (a) JUNB mRNA expression in HTR-8/SVneo cells transfected with Vector and oe-JUNB was measured by RT-qPCR. (b) JUNB mRNA expression in HTR-8/SVneo cells transfected with si-NC, si-circPUM1, si-circPUM1+ Vector, si-circPUM1+ oe-JUNB was measured by RT-qPCR. (c) Cell proliferation was analyzed by CCK-8 assay. (d and e) Transwell assay was conducted to detect cell migration and invasion abilities. (f) Cell apoptosis was analyzed using Flow cytometry analysis. (g) The cleaved caspase-3 level was detected by Western blotting. (h-j) TNF-α (h), IL-6 (i), and IL-8 (j) expressions were determined by ELISA. All data were obtained from three independent experiments (n = 3). *P < 0.05 and **P < 0.01