Figure 3.
PTHrP inhibited FOS-dependent kinase phosphorylation by mediating MKP1 in HPdLFs. PTHrP reduced FOS-induced ERK1/2 activation in HPdLFs, and the effect was suppressed by MKP1 inhibitor, sanguinarine (0.5 mM) (a). PTHrP reduced FOS-induced p38 activation in HPdLFs, and the effect was suppressed by MKP1 inhibitor, sanguinarine (0.5 mM) (b). PTHrP reduced FOS-induced p66Shc activation in HPdLFs, and the effect were suppressed by MKP1 inhibitor, sanguinarine (0.5 mM) (c). Data represented mean±standard error of mean (SEM) of six independent experiments. *P < 0.05 versus control; aP<0.05 versus FOS stimulation; bP<0.05 versus corresponding condition without inhibitor