Figure 3.

HOXA-AS3 directly interacted with miR-218-5p. (a) Predicted binding of human miR-218-5p with the wild-type 3ʹUTR region of HOXA-AS3 mRNA and a mutated 3ʹUTR of HOXA-AS3. (b, c) Luciferase reporter gene assay verified that HOXA-AS3 could directly bind to miR-218-5p in SCC-9 and CAL-27 cells. (d, e) SCC-9 and CAL-27 cells were transfected with miR-218-5p mimics or control, followed by the measurement of HOXA-AS3 mRNA enrichment with anti-Ago2 by qRT-PCR, and anti-IgG used as control. *P < 0.05, **P < 0.01