Figure 2.

Chrysophanol induced cell cycle arrest and increased Cytosol Cyt C expression in glioma cells. (a) Cell cycle of U251 and SHG-44 cells was tested by flow cytometry after treatment with different concentration of chrysophanol. (b) Representative images of cell cycle in U251 and SHG-44 cells using flow cytometry after treatment with different concentration of chrysophanol. (c) Representative images of Cytosol Cyt C protein bands through western blot after treatment with different concentration of chrysophanol. β-actin was used as a loading control. (d) Cytosol Cyt C protein expression levels of U251 and SHG-44 cells were detected by western blot after treatment with different concentration of chrysophanol. β-actin was used as a loading control. *p < 0.05, **p < 0.01, *** p < 0.001 vs. Control group. All experiments were repeated independently at least three times. Data were expressed as the means ± standard deviation