Figure 2.

Upregulation of miR-1224-5p attenuated TNF-α-induced secretion of androgens and inflammation in human granulosa-like tumor (KGN) cells. KGN cells were treated with TNF-α for 24 h to establish an in vitro study model. The levels of (a) DHEA and (b) testosterone in TNF-α-treated KGN cells versus controls. (c) The relative mRNA expression of miR-1224-5p following TNF-α treatment. To investigate the role of miR-1224-5p, KGN cells were transfected with miR-1224-5p mimics or negative control (NC) mimics, followed by TNF-α treatment. (d) The relative mRNA expression of miR-1224-5p was determined to verify the efficiency of transfection. (e) The relative mRNA expression of miR-1224-5p in KGN cells with different treatments. The levels of (f) DHEA, (g) testosterone and (h) IL-1β in KGN cells with different treatments. Data were shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 versus Control group. ++p < 0.01, versus TNF-α+ NC mimics group