Figure 3.

Upregulation of miR-1224-5p inhibited the activation of NLRP3 inflammasome in TNF-α-treated KGN cells. miR-1224-5p was enforced expressed in KGN cells with miR-1224-5p mimics. After 24-h transfection, cells were induced with TNF-α for 24 h. (a) The protein bands and (b) the relative protein expression of NLRP3, ASC, pro-caspase-1 and cleaved caspase-1 in KGN cells. The relative mRNA expression of (c) NLRP3 and (d) ASC in KGN cells. (e) The level of caspase-1 in KGN cells quantified by the ELISA kit. (f) The protein bands and (g) the relative protein expression of FOXO1 and thioredoxin interaction protein (TXNIP) in KGN cells. (h) The relative mRNA expression of TXNIP in KGN cells. Data were shown as mean ± SD (n = 3). ***p < 0.001, versus Control group. ++p < 0.01, +++p < 0.001 versus TNF-α+ NC mimics group