Figure 5.

miR-1224-5p inhibited the activation of NLRP3 inflammasome in KGN cells by targeting FOXO1. To investigate the involvement of FOXO1 in the regulation of miR-1224-5p, FOXO1 was enforced upregulated in KGN cells with miR-1224-5p overexpression. (a) Predicted binding site of miR-1224-5p in wild-type FOXO1 3ʹUTR and mutant sites. Luciferase reporter assay was performed in HEK-293 T cells. Following different treatment, (b) the relative mRNA expression of miR-1224-5p in KGN cells. (c-d) The relative mRNA and protein expression of FOXO1 in KGN cells. (e) The protein band and (f) the relative protein expression of NLRP3, ASC, pro-caspase-1 and cleaved caspase-1 in KGN cells. (g) ELISA analysis of IL-1β level in KGN cells. Data were shown as mean ± SD (n = 3). ###P < 0.001, versus NC mimics+FOXO1 3ʹUTR (Wt) group or miR-1224-5p mimics+FOXO1 3ʹUTR (Mut) group. **p < 0.01, ***p < 0.001 versus TNF-α+ NC mimics group. ++p < 0.01, +++p < 0.001 versus TNF-α+ miR-1224-5p mimics+NC vector group