Figure 2.

Overexpression of hsa_circ_0005567 inhibits M1 polarization and promoted M2 polarization. Human monocyte THP-1 was cultured in vitro and induced to differentiate into macrophages. Then THP-1 macrophages were randomly divided into 4 groups: ① PBS group, ② LPS (100 ng/mL) group, ③ Vector+ LPS group, ④ hsa_circ_0005567 overexpression plasmid + LPS group. (a) The overexpression efficiency of hsa_circ_0005567 in THP-1 macrophage was detected by qRT-PCR. (b) The hsa_circ_0005567 expression was decreased by LPS treatment. (c) Overexpression of hsa_circ_0005567 reversed LPS-induced M1 macrophage markers expression, and promoted M2 macrophage marker expression. (d-f) Overexpression of hsa_circ_0005567 reversed LPS-increased proportion of M1 macrophage, and increased proportion of M2 macrophage. **p < 0.01 vs vector group, ^##p < 0.01 vs Control group, ^$$p < 0.01 vs Vector+LPS group