Figure 4.

Hsa_circ_0005567 regulates macrophage polarization by regulating miR-492/SOCS2 signal axis.THP-1 macrophages were randomly divided into four groups:① vector+mimic NC group,② Hsa_circ_0005567 overexpression plasmid+mimic NC group,③vector+miR-492 mimic group,④ Hsa_circ_0005567 overexpression plasmid+ miR-492 mimic group, followed by LPS treatment. (a-b) Overexpression of hsa_circ_0005567 upregulated the mRNA and protein expression of SOCS2, which was inhibited by miR-492 mimic. (c) Overexpression of hsa_circ_0005567 inhibited the expression of M1 macrophage marker and promoted the expression of M2 macrophage markers, which was reversed by miR-492 mimic. (d-f) Overexpression of hsa_circ_0005567 reduced the proportion of M1 macrophage and increased the proportion of M2 macrophage, which was reversed by miR-492 mimic. **p < 0.01 vs vector + mimic NC group, ^##p < 0.01 vs hsa_circ_0005567+ mimic NC group