Figure 5.

Effect of hsa_circ_0005567-regulate macrophage polarization on chondrocyte viability and apoptosis. Human chondrocytes were cultured in vitro and randomly divided into 5 groups: ① Control group, ② PBS group, ③ LPS group, ④ Vector+LPS group, ⑤ hsa_circ_0005567 overexpression plasmid + LPS group. (a) The chondrocyte viability was reduced in LPS group, but overexpression of hsa_circ_0005567 increased chondrocytes viability. (b) The chondrocyte apoptosis ratio was increased in LPS group, but overexpression of hsa_circ_0005567 decreased chondrocytes apoptosis. (c) The supernatant from LPS-treated macrophage significantly increased the expression of apoptotic proteins and decreased the expression of anti-apoptotic protein, which were reversed by hsa_circ_0005567 overexpression. **p < 0.01 vs PBS group, ^##p < 0.01 vs vector+ LPS group