Figure 2.

Circ_0054537 deletion boycotted cancer properties of RCC cells in vitro. (a, b) RT-qPCR compared circ_0054537 level between si-con transfection and si-circ_0054537 (^{#1, #2} and ^#3) transfection in 786-O and A498 cells. (c, d) CCK-8 assay compared OD values, (e, f) transwell assays compared migration cell number, (g, h) transwell assays with Matrigel compared invasion cell number, (I, J, M, N) western blotting compared protein levels of LC3 II/I, MMP-9 and Bax, (k, l) FCM compared apoptotic rate (%), (o, p) XF96 extracellular flux analyzer compared ECAR (mpH/minute), and (q, r) ATP assay Kit compared ATP level between si-con transfection and si-circ_0054537^#1 transfection in 786-O and A498 cells. **P < 0.01 and ***P < 0.001