Figure 6.

NPTX2 upregulation abrogated the tumor-suppressive role of miR-640 in RCC cells in vitro. (a) RT-qPCR detected miR-640 level and (b-d) western blotting detected PTX2 protein level in 786-O and A498 cells transfected with miR-con, miR-640, pcDNA vector, or pcDNA-NPTX2 (NPTX2) vector, and co-transfected with miR-640 and pcDNA or NPTX2 vector. (e, f) CCK-8 assay compared OD values, (g-j) transwell assays compared migration cell number and invasion cell number, (K, L, O, P) western blotting compared LC3 II/I protein level, (m, n) FCM compared apoptotic rate (%), (q, r) XF96 extracellular flux analyzer compared ECAR (mpH/minute), and (s, t) ATP assay Kit compared ATP levels between transfections of si-con and si-circ_0054537^#1, and co-transfections of si-circ_0054537^#1 with in-miR-con or in-miR-640 in 786-O and A498 cells. *P < 0.05, **P < 0.01 and ***P < 0.001