Figure 1.

MiR–143–3p is lifted in RPL and impairs the biological functions of trophoblastic cells. (a) Relative miR–143–3p expression in placental tissues from RPL patients (n = 28) and normal pregnant women (n = 28) was detected by RT-qPCR. (b) miR–143–3p expression in HTR8/SVneo cells transfected with NC mimics, NC inhibitor, or miR–143–3p inhibitor was detected by RT-qPCR. (c) The cell viability of transfected HTR8/SVneo cells was analyzed by CCK-8 assay at different time points. (d) The apoptosis condition of transfected HTR8/SVneo cells was analyzed by flow cytometry assay. (e) The migration capability of transfected HTR8/SVneo cells was analyzed by wound healing assay. (f) The invasion capability of transfected HTR8/SVneo cells was analyzed by transwell assay. (g and h) The mRNA or protein expression level of EMT markers (E-cadherin, vimentin, and N-cadherin) in transfected HTR8/SVneo cells was determined by RT-qPCR or western blotting. Data are shown as the mean ± SD (n = 3). *P < 0.05; **P < 0.01