Figure 2.

S100A11 is directly targeted by miR–143–3p in trophoblastic cells. (a) 8 candidate target mRNAs for miR–143–3p were predicted by using three miRNA databases (miRanda, miRmap, and PITA). (b) Relative S100A11 expression in placental tissues from RPL patients (n = 28) and normal pregnant women (n = 28) was detected by RT-qPCR. (c) The putative binding sites between miR–143–3p and S100A11. (d) The HTR8/SVneo cells were co-transfected with either S100A11-WT or S100A11-MUT, and miR–143–3p mimics/inhibitor or corresponding NC. Then, the relative luciferase activity was measured. (e) Pearson’s correlation analysis indicated a negative correlation between the expression of miR–143–3p and S100A11 in placental tissues from RPL patients (n = 28). (f and g) S100A11 mRNA or protein level in HTR8/SVneo cells transfected with NC mimics, miR–143–3p mimics, NC inhibitor or miR–143–3p inhibitor was detected by RT-qPCR or western blotting. Data are shown as the mean ± SD (n = 3). *P < 0.05; **P < 0.01