Figure 5.

CircFOXP1/miR–143–3p/S100A11 axis regulates trophoblastic cell functions. (a) HTR8/SVneo cells transfected with vector, oe-circFOXP1, oe-circFOXP1+NC mimics, or oe-circFOXP1+ miR–143–3p mimics, respectively. The cell viability of transfected HTR8/SVneo cells was analyzed by CCK-8 assay at different time points. (b) The apoptosis condition of transfected HTR8/SVneo cells was analyzed by flow cytometry assay. (c) The migration capability of transfected HTR8/SVneo cells was analyzed by wound healing assay. (d) The invasion capability of transfected HTR8/SVneo cells was analyzed by transwell assay. (e and f) The mRNA or protein expression level of S100A11 and EMT markers (E-cadherin, vimentin, and N-cadherin) in transfected HTR8/SVneo cells were determined by RT-qPCR or western blotting. data are shown as the mean ± SD (n = 3). *P < 0.05; **P < 0.01