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. 2022 Jan 25;2022:2785113. doi: 10.1155/2022/2785113

Figure 3.

Figure 3

TIMP3 is a target of miR-17-3p. (a) The miR-17-3p binding site in the TIMP3 3′UTR was predicted using TargetScan7.2. (b) Dual-luciferase reporter assay was used to detect the luciferase activity cotransfected with 3′UTR of TIMP3 with the wild-type binding site or mutant binding site and miR-17-3p mimic. (c) The miR-17-3p targets were predicted with TargetScan7.2. (d) ZNF28, FAM83F, Neurog1, and TIMP3 levels in the cardiac I/R injury model were determined by Western blotting. Data are presented as the mean ± SD; ∗∗P < 0.01, model vs. control. (e) The effect of miR-17-3p on ZNF28, FAM83F, Neurog1, and TIMP3 levels was determined by Western blot analysis. The data are presented as the mean ± SD (n = 3); ∗P < 0.05; ∗∗∗∗P < 0.001.