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TIMP3 affected H2O2-induced programmed necrosis of primary cardiomyocytes and was regulated by miR-17-3p in vitro. (a) Necrotic cell death was assessed using PI staining of H9C2 cells following TIMP3 overexpression or knockdown. (b) The function of TIMP3 in necrotic cell death was identified using a rescue experiment. Representative images show PI-positive cells (bar = 100 μm). Data are presented as the mean ± SD (n = 3); ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
