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Atg5, Atg12, LC3B, and Beclin1 were used as ovarian autophagy-related genes. mRNA expression levels were confirmed and compared through quantitative RT-PCR. There was no significant change in mRNA expression levels of Atg5, Atg12, LC3B, and Beclin1 genes. All data are represented as means±SEM. OS, once stimulation; RS, repeated stimulation.
