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. 2021 Aug 2;36(2):348–360. doi: 10.1038/s41375-021-01361-8

Fig. 1. One-carbon folate metabolism is a dependency in T-ALL.

Fig. 1

A Volcano plots showing enrichment of KEGG pathways for 689 cell lines in the Avana 19Q4 data set. The KEGG one-carbon pool by folate, purine and pyrimidine metabolism pathways scored as most depleted in the T-ALL lineage (n = 3) compared to all other cell lines (n = 686) (left) (P = 0.0003, Mann–Whitney test) and to other hematopoietic cell lines (n = 73) (right) (P = 0.025, Mann–Whitney test). Normalized enrichment score (NES) shown on X-axis. B Graph showing the distribution of the ssGSEA Z-scores for the one-carbon pool by folate pathway across cancer cell lineages represented in the Avana 19Q4 data set. The one-carbon pool by folate pathway is significantly enriched in T-ALL vs. non-T-ALL hematopoietic (*P ≤ 0.05, Mann–Whitney test) and T-ALL vs. solid tumor (***P ≤ 0.001, Mann–Whitney test) cell lines. C Heatmap of ssGSEA projections for the primary ALL sample data set GSE33315 on the collection of KEGG canonical pathways. T-ALL samples are highlighted in red.