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. 2021 Aug 31;36(2):370–382. doi: 10.1038/s41375-021-01392-1

Fig. 4. Phf6 deletion increases the activity of LICs in T-ALL mice.

Fig. 4

A Schematic representation of secondary transplantation. B, C Percentage of GFP+ leukemia cells in the PB and BM of mice at 8 weeks after secondary transplantation. D Kaplan–Meier survival curves of VC Phf6 + JAK3M511I T-ALL mice and Phf6 WT + JAK3M511I T-ALL mice in the secondary transplantation assay (log-rank test P < 0.0001, n = 10 per group). E Percentage of T cells, B cells and myeloid cells in GFP+ leukemia cells in the BM of mice at 8 weeks after secondary transplantation. F Percentage of GFP+ CD25+ cells in GFP+ leukemia cells in the BM of Phf6 KO + JAK3M511I mice and Phf6 WT + JAK3M511I mice. G The expression of TCR-β in GFP+CD8+cells of Phf6 KO + JAK3M511I mice and Phf6 WT + JAK3M511I mice. H, I Phf6 KO + JAK3M511I mice had more LICs (P < 0.0001) according to the extreme limiting dilution assay (n = 10 per dose). J LIC analysis in mice transplanted with Phf6 knockout and wild-type leukemia cells. Confidence intervals showing 1/ (stem cell frequency) based on H.