FIGURE 5.

Mild protection of EAG optic nerves. (A) Optic nerves were stained with H&E and LFB. (B) The H&E score revealed no signs of inflammation within all groups. (C) Also, no disruption of myelin could be observed via the LFB score in all groups. (D) The neurofilament was labelled with an antibody against SMI-32 (green) and DAPI stained cell nuclei (blue). (E) A disruption of neurofilaments could be detected in ONA optic nerves via SMI-32 score. No changed were noted in ONA + ATX-i animals compared to controls. (F) The total number of microglia was labelled with an antibody against Iba1 (green) and activated microglia were additionally stained with ED1 (red). DAPI counterstained cell nuclei (blue). (G) Significantly more Iba1⁺ microglia were noted in ONA and in ONA + ATX-i optic nerves. (H) However, no alterations in regard to active microglia were noted within all groups. (I) Astrocytes were stained against anti-GFAP (red), while DAPI labelled cell nuclei (blue). (J) The GFAP score in ONA as well as ONA + ATX-i optic nerves was not altered in comparison to control ones. Values are mean ± SD. 5-6 nerves/group. Scale bars: 20 μm **p < 0.01.