Fig. 2.

Anlotinib suppresses the migration and invasion capacity of glioblastoma cells in vitro. a A172, U251 and U87 cells were treated with vehicle or anlotinib for 24 h. Cells that migrated into the wounded areas were observed and imaged. b Quantification of the wound-healing ability. c, e The migration and invasion of glioblastoma cells in Transwell migration and Matrigel invasion assays were inhibited. d, f Quantification of the number of migrated and invasive cells was analyzed from five independent fields of view. The wound-healing ability of the vehicle-treated group was adjusted to the value of 100. Values are expressed as the mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control