Fig. 5.

The combination of anlotinib and TMZ enhances the effect of anti-glioblastoma cells. a The combination of anlotinib and TMZ enhanced the effect of suppressing growth of human glioblastoma cell lines (U87 and U251), compared with single drug treatments. b, c Anlotinib combined with TMZ greatly suppresses the migration capacity of glioblastoma cells. d, e The combination treatment enhanced the cytotoxic effects in human glioblastoma cell lines. f, g The suppressive effect on the JAK2/STAT3/VEGFA pathway was enhanced with the combination of drugs. The relative protein levels in control cells were adjusted to the value of 1. Data are represented as mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs control group, ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 vs anlotinib group