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. 2022 Jan 19;12:827256. doi: 10.3389/fimmu.2021.827256

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Copyright © 2022 Chang, Krenger, Krueger, Zha, Han, Yermanos, Roongta, Mohsen, Oxenius, Vogel and Bachmann

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fel d 1-specific antibody responses of WT mice upon Qβ_RNA-Fel d 1 or Qβ_empty-Fel d 1 immunization. (A) Immunization scheme. Fourteen mice were immunized with 30 μg Qβ_RNA-Fel d 1 or Qβ_empty-Fel d 1 at d0 and were bled at d7. Eight mice were euthanized to harvest spleen and sort Fel d 1-specific B cells at d10, so 6 mice were bled at d14 and euthanized for Fel d 1-specific B cells sorting at d17. Fel d 1-specific antibody titers (B), avidity indexes (C), and IgG2b (D), IgG2c (E) and IgG1 (F) subclasses generated by WT mice after immunization were determined by ELISA. p value from unpaired t-test was indicated as ≤0.001 (***), ≤0.0001 (****). The figure is based on one experiment.