Fig. 5.

Effect of 1E10 mAb on the rLF enzymatic activity towards MEK1 and MEK2. (A) – Western blot results correlated with molecular weight markers. (B) – Enlarged image of Western blot results. 1 – Molecular weight markers SM0671 (Fermentas, USA); 2 – Control: intact J774A.1 cells; 3 – J774A.1 cells + LT, incubation for 30 min; 4 – J774A.1 cells + (LT + 1E10 mAb), incubation for 30 min; 5 – J774A.1 cells + LT, incubation for 60 min; 6 – J774A.1 cells + (LT + 1E10), incubation for 60 min; 7 – J774A.1 cells + LT, incubation for 120 min; 8 – J774A.1 cells + (LT + 1E10), incubation for 120 min; 9 – J774A.1 cells + LT, incubation for 240 min; 10 – J774A.1 cells + (LT + 1E10), incubation for 240 min