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. 2022 Jan 19;9:801422. doi: 10.3389/fcell.2021.801422

FIGURE 5.

FIGURE 5

NLRP3 knockout alleviates sevoflurane-induced pyroptosis. (A) Western blotting analysis of cleaved gasdermin D (GSDMD), cleaved caspase-1, IL-1β, and IL-18 induced by sevoflurane in wild-type (WT) and NLRP3−/− BV2 cells. Protein band intensity was quantified using ImageJ software. (B) Immunoblot analysis of p-tau, tau-correlated kinases, and phosphatases induced by sevoflurane in WT and NLRP3−/− BV2 cells. (C) FAM-FLICA caspase-1 assay of cleaved caspase-1 (green) induced by sevoflurane in WT and NLRP3−/− BV2 cells. Scale bar: 50 μm (D). Quantitation of cleaved caspase-1 fluorescence intensity as iod. (E). Lactate dehydrogenase (LDH) release and Cell Counting Kit-8 (CCK-8) assays of LDH activity and cell viability induced by sevoflurane in WT and NLRP3−/− BV2 cells. ***p < 0.001, **p < 0.01, *p < 0.05.