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. 2021 Nov 15;32(2):190–209. doi: 10.1038/s41422-021-00584-9

Fig. 5. MTs that penetrate the nucleus in C11ORF49/CSTPP1 KO cells drive lobulation.

Fig. 5

a MT organization was examined in control and C11ORF49/CSTPP1−/− telophase cells using correlative light and electron microscopy (CLEM). IF images and correlative EM images are shown with and without pseudo-color (blue for nucleus, red for MT). Arrows indicate Alexa-546/nano-gold-labeled nuclear-penetrating MTs captured by immuno-EM and conventional microscopy. Schematic of CLEM method used in this study and serial-section EM images of the same nuclei are included in Supplementary information, Fig.S6c. (Right) Cartoons indicate the position of MT based on serial section EM images. Scale bars = 2 μm. b Control and C11ORF49/CSTPP1−/− RPE1 cells were incubated with nocodazole (10 μM) for 4 h and stained with DAPI to examine nuclear shape. n ≥ 100 per sample were analyzed in three independent experiments. Nuclei with >2 lobes were defined as lobulated or multi-lobed. n ≥ 40 per sample were analyzed in three independent experiments in b. Circularity of nuclei was measured with CellProfiler software Form factor function. All data are presented as means ± SD. *P < 0.05 (unpaired t-test or Mann-Whitney test). Scale bars = 10 μm.