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. 2022 Feb 1;13:603. doi: 10.1038/s41467-022-28293-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Upper panel: communities exposed to DTM and CM; lower left panel: communities exposed to filtered CM on both sides; lower right panel: communities exposed to dialysed CM (12 kDa cut-off) or undialysed CM. White circles with dots indicate where solutions were pipetted onto the plates. Community lifts stained for EP (green) and GPEET (red) are shown. b Continuous growth in liquid culture acidifies medium. Representative pictures of culture supernatants from early procyclic forms (EATRO1125) grown in DTM for 7 days without dilution. Left, DTM only. Samples were taken on days 0, 4, and 7. The colour change from red to yellow indicates that the culture becomes more acidic with time. Cell titres are indicated.