| Time (min) | Flow rate (mL/min) | %A | %B | Curve |
|---|---|---|---|---|
| Initial | 0.300 | 90.0 | 10.0 | Initial |
| 1.00 | 0.300 | 90.0 | 10.0 | 6 |
| 3.00 | 0.300 | 10.0 | 90.0 | 6 |
| 4.00 | 0.300 | 10.0 | 90.0 | 6 |
| 4.10 | 0.300 | 90.0 | 10.0 | 6 |
| 5.00 | 0.300 | 90.0 | 10.0 | 6 |
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