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. 2022 Jan 19;12:818227. doi: 10.3389/fphar.2021.818227

FIGURE 1.

FIGURE 1

The effects of POL in the treatment of NASH in MCD diet-induced mouse model. Male C57BL/6 mice were fed with MCD diet for 6 weeks to establish a NASH model and treated with 500 mg/kg POL or 30 mg/kg PGZ for 3 weeks (A). HE and oil red O staining (ORO) were performed to observe the histopathological characteristics of liver tissue after the treatment of POL (B). Then, the NAS score (G), including hepatic steatosis (D), lobular inflammation (E), and ballooning degeneration (F), were calculated. The specific classification criteria were as follows: 1) hepatic steatosis (<5%, 0 points; 5–33%, 1 point; 33–66%, 2 points; >66%, 3 points); 2) lobular inflammation (none, 0 points; < 2 lesions/200× view, 1 point; 2–4 lesions/200× view, 2 points; > 4 lesions/200× view, 3 points); and 3) ballooning degeneration (none, 0 points; rare, 1 point; extensive, 2 points). The contents of hepatic TG (C), serum ALT (H), AST (I), TC (J), and TG (K) were detected. Comparing with model group, *p < 0.05, **p < 0.01, ***p < 0.001.