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. 2022 Jan 19;11:771483. doi: 10.3389/fcimb.2021.771483

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Copyright © 2022 Yin, Liu, Jiang, Zhang, Wei and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparisons of gene expression profiles in the rat liver by RNA-seq among different treatment groups. (A) Principal component analysis showed that the NC group had distinct gene expression signatures compared with the ALF rats and QCLGF rats PC1 (explaining 77.68% of variation). (B) Heatmap of DEGs. Compared with the NC group, the number of differentially expressed genes (DEGs) in the ALF groups was 13553 (DEG1, 529/13024; upregulated and downregulated DEGs, DEGseq, respectively). Genes of QCLGF vs. ALF and NC vs. ALF with the same trend were 1610 (DEG3, 1581/29; upregulated and downregulated DEGs, DEGseq, respectively), DEG3 genes were further clustered into 10 QCLGF clusters (QCs) according to their expression profiles. (C) KEGG annotation of DEG3. (D) GO annotation analysis of DEG3.