Figure 1.
RIOK3 mRNA is alternatively spliced during RVFV infection in multiple cell types. (A) Schematic illustration of RIOK3 splice sites observed. Single lines are introns, while boxes are exons. (B) RT-PCR visualized by agarose gel showing alternative splicing of RIOK3 pre-mRNA after infection with RVFV MP-12 at MOI = 1. (C) RT-PCR showing RIOK3 alternative splicing in multiple cell lines in response to RVFV MP-12 infection. HEK293 (human embryonic kidney), Hela (human cervical carcinoma), HEPG2 (human hepatoma), HC-04 (human hepatoma), Vero (African green monkey kidney), SH-SY5Y (human neuroblastoma), and HFF (human foreskin fibroblast) were used.