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. 2022 Feb 1;13:590. doi: 10.1038/s41467-022-28257-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Release of SCC from the sacculi purified from S. mutans strains by mild acid hydrolysis (left) or deamination with HONO (right). b Release of the Rha-containing polysaccharides from the cell wall purified from GBS, S. equi and S. thermophilus strains by mild acid hydrolysis (left) or deamination with HONO (right). The concentration of polysaccharide released from the sacculi or cell wall was estimated by the modified anthrone assay and normalized to total content of polysaccharide in the starting material. Symbols and error bars represent the mean and S.D., respectively (n = 3 biologically independent replicates). P values were calculated by two-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source data file.