Representative chromatograms of recreational C. sativa extract. a Unspiked recreational cannabis extract (1 mg/ml). The extract was spiked with 20 µl (1 mg/ml) of commercial cannabinoid standards to identify and quantify the presence of the major and minor cannabinoids of interest. Cannabinoid standards included: b CBG, c CBD, d CBN and e THC. Retention times (in minutes) for CBG, CBD, CBN and THC were 3.15, 3.30, 5.00, and 6.26 min, respectively