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. Author manuscript; available in PMC: 2023 Feb 17.
Published in final edited form as: Cell Chem Biol. 2021 Aug 6;29(2):328–338.e4. doi: 10.1016/j.chembiol.2021.07.013

Figure 3. Engineered PriSMs exhibit potent, isoform-specific inhibition.

Figure 3.

(A) 4-nitrophenyl acetate is converted to 4-nitrophenol and acetic acid by carbonic anhydrase. The ability of PriSMs to inhibit this activity is titrated by spectrophotometrically measuring 4-nitrophenol production over time at different PriSM concentrations. (B) Titration curves of FnII-28.7-PEG7-AAZ (solid lines) and PEG7-AAZ (dashed lines) with CA-II and CA-IX. (C) Inhibition constants. Data are presented as the mean ± 68% confidence interval for three replicates. Inhibition was not detected for FnII-28.7.