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. Author manuscript; available in PMC: 2023 Feb 17.
Published in final edited form as: Cell Chem Biol. 2021 Aug 6;29(2):328–338.e4. doi: 10.1016/j.chembiol.2021.07.013

Figure 5. The PriSM-enzyme interface is finely tuned to linker length.

Figure 5.

(A,B) Modifying the linker from the length of original discovery hinders specificity. Enzyme inhibition constants for FnII.28.7-Lx-AAZ (A) and FnII.80.2-Lx-AAZ (B) (and both Fn proteins alone) are presented as the mean ± 68% confidence interval of triplicate measurements. Inhibition was not detected for Fn without conjugation. (C) Relative frequency across different linker length campaigns for CA-II binders from Cys80 library. Data exist at lengths 2, 3, 5, and 7; connecting lines are drawn for visual guidance.