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. Author manuscript; available in PMC: 2023 Feb 17.
Published in final edited form as: Cell Chem Biol. 2021 Aug 6;29(2):328–338.e4. doi: 10.1016/j.chembiol.2021.07.013

Figure 6. PriSM sequence landscape.

Figure 6.

(A) The Hamming distance between all pairs of variants in each population. Black represents the unsorted libraries. (B) The relative frequency of each variant in each population. (C) The relative frequency of each loop length for each campaign (Cys28 or Cys80 conjugation sites; naïve library or sorted for binding in the presence of conjugates with linkers of PEG2, PEG3, PEG5, or PEG7. (D) Sitewise amino acid enrichment, from the naïve library to the sorted binding population, is presented for each target (CA-II or CA-IX) and conjugation site (Cys28 or Cys80) combination, with results from all linker lengths aggregated. Sites with possible gaps for loop length diversity are underlined.