Antibodies
|
|
|
Mouse c-MYC Monoclonal Antibody (9E10) |
Invitrogen |
Cat# MA1-980, RRID AB_558470 |
Streptavidin, Alexa Fluor™ 488 conjugate |
Invitrogen |
Cat# S11223
|
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary antibody, PE |
Invitrogen |
Cat# P-852, RRID AB_2539848 |
Bacterial and Viral Strains
|
|
|
Escherichia coli: DH5α |
New England Biolabs |
Cat# C2987C |
Escherichia coli: T7 Express |
New England Biolabs |
Cat# C2566H |
Chemicals, Peptides, and Recombinant Proteins
|
|
|
4-Nitrophenyl acetate |
Sigma-Aldrich |
Cat# N8130-5G |
DyLight™ 650 NHS Ester, 5 x 50 ug |
Thermo Scientific |
Cat# 62266 |
EZ-Link™ Sulfo-NHS-LC-Biotin |
Thermo Scientific |
Cat# 21335 |
HisPur™ Cobalt Resin |
Thermo Scientific |
Cat# 89964 |
Human Carbonic Anhydrase II Protein (Recombinant) |
LifeSpan BioSciences, Inc |
Cat# LS-G551-100 |
Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His Tag |
ACRO Biosystems |
Cat# CA9-H5226-1 mg |
Carbonic Anhydrase IX Protein, Human, Recombinant (His Tag) |
Sino Biological Inc. |
Cat# 10107-H08H |
Maleimide-PEG2-acetazolamide |
Peptech |
N/A |
Maleimide-PEG3-acetazolamide |
Peptech |
N/A |
Maleimide-PEG5-acetazolamide |
Peptech |
N/A |
Maleimide-PEG7-acetazolamide |
Peptech |
N/A |
Critical commercial assays
|
|
|
Zymoclean Gel DNA Recovery Kit |
Zymo Research |
Cat# D4002 |
Zeba Spin Desalting column, 7 kDa |
ThermoFisher |
Cat# 89882 |
LongLife Zymolyase |
G-Biosciences |
Cat#: 786-036 |
Experimental models: cell lines
|
|
|
Saccharomyces cerevisiae: EBY100 |
ATCC |
MYA-4941 |
Oligonucleotides
|
|
|
See Table S1 for PCR primers used in this study |
|
|
Recombinant DNA
|
|
|
pCT40-FnLoopHP |
Woldring et al., 2015
|
N/A |
pET-24b |
Lipovšek et al., 2007 (Novagen) |
N/A |
Software and Algorithms
|
|
|
BioTek Gen5 Software Version 2.09 |
Biotek |
https://www.biotek.com/products/software-robotics/
|
Matlab R2020a |
MathWorks |
https://www.mathworks.com/
|
PyMol 2.4.0 Open-Source |
PyMol |
https://www.pymol.org/
|
Python 3.8.8 |
Python |
https://www.python.org/
|
Code / Scripts |
This manuscript |
https://github.com/HackelLab-UMN
|