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. Author manuscript; available in PMC: 2023 Feb 17.
Published in final edited form as: Cell Chem Biol. 2021 Aug 6;29(2):328–338.e4. doi: 10.1016/j.chembiol.2021.07.013

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse c-MYC Monoclonal Antibody (9E10) Invitrogen Cat# MA1-980, RRID AB_558470
Streptavidin, Alexa Fluor™ 488 conjugate Invitrogen Cat# S11223
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary antibody, PE Invitrogen Cat# P-852, RRID AB_2539848
Bacterial and Viral Strains
Escherichia coli: DH5α New England Biolabs Cat# C2987C
Escherichia coli: T7 Express New England Biolabs Cat# C2566H
Chemicals, Peptides, and Recombinant Proteins
4-Nitrophenyl acetate Sigma-Aldrich Cat# N8130-5G
DyLight™ 650 NHS Ester, 5 x 50 ug Thermo Scientific Cat# 62266
EZ-Link™ Sulfo-NHS-LC-Biotin Thermo Scientific Cat# 21335
HisPur™ Cobalt Resin Thermo Scientific Cat# 89964
Human Carbonic Anhydrase II Protein (Recombinant) LifeSpan BioSciences, Inc Cat# LS-G551-100
Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His Tag ACRO Biosystems Cat# CA9-H5226-1 mg
Carbonic Anhydrase IX Protein, Human, Recombinant (His Tag) Sino Biological Inc. Cat# 10107-H08H
Maleimide-PEG2-acetazolamide Peptech N/A
Maleimide-PEG3-acetazolamide Peptech N/A
Maleimide-PEG5-acetazolamide Peptech N/A
Maleimide-PEG7-acetazolamide Peptech N/A
Critical commercial assays
Zymoclean Gel DNA Recovery Kit Zymo Research Cat# D4002
Zeba Spin Desalting column, 7 kDa ThermoFisher Cat# 89882
LongLife Zymolyase G-Biosciences Cat#: 786-036
Experimental models: cell lines
Saccharomyces cerevisiae: EBY100 ATCC MYA-4941
Oligonucleotides
See Table S1 for PCR primers used in this study
Recombinant DNA
pCT40-FnLoopHP Woldring et al., 2015 N/A
pET-24b Lipovšek et al., 2007 (Novagen) N/A
Software and Algorithms
BioTek Gen5 Software Version 2.09 Biotek https://www.biotek.com/products/software-robotics/
Matlab R2020a MathWorks https://www.mathworks.com/
PyMol 2.4.0 Open-Source PyMol https://www.pymol.org/
Python 3.8.8 Python https://www.python.org/
Code / Scripts This manuscript https://github.com/HackelLab-UMN