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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Gut. 2021 Aug 2;71(6):1161–1175. doi: 10.1136/gutjnl-2021-325288

Figure 1: Cytokines in the tumor environment of patients with HCC.

Figure 1:

A. Heatmap showing expression of cytokines, cytokine receptors and growth factors by individual region analyzed by bulk tissue RNA sequencing from liver and tumor tissue of patients with HCC. Cytokine expression data is derived from 59 samples from a total of 25 patients. Matched non-tumor (NT), margin (M) and tumor (T) samples were available from 11 patients, matched NT and M from 11 patients and NT only from 3 patients.

B. Multiplex cytokine analysis of supernatants derived from NT (black) or T tissue (red). Cytokine concentration in supernatants harvested from culturing of minced non-tumor or tumor tissue, incubated for 6h and cleared from residual cells. Adjusted for weight of tissue piece. 6 matched NT and T samples with two additional T samples. Bar graphs presenting median with 95% confidence interval.

C. Expression of TGFB2 comparing NT, M and T in the HCC cohort.

D. Expression of IL33 comparing NT, M and T in the HCC cohort.

E. Expression of IL1B comparing NT, M and T in the HCC cohort.

F. Survival prediction analysis of TCGA liver cancer cohort using expression of IL33. Patients were split at median.