Skip to main content
. 2022 Feb 1;13:597. doi: 10.1038/s41467-022-28300-0

Fig. 4. Expanding the application of Ades to rA1-CBE and ABE8e.

Fig. 4

a Schematic representation of rA1-, A3G-, A3Gntd-rA1- and dA3G-rA1-CBEs. nCas9, D10A. b, c C-to-T editing of seven CBEs in the presence or absence of Ade3 and Ade4 at the EMX1-1 site (b) and FNACF site (c). Plasmids expressing CBE, sgRNA, and each Ade (1:1:1) were cotransfected into HEK293T cells. d Top: Schematic representation of dA3G-ABE8e. Bottom: A-to-G editing of ABE8e and dA3G-ABE8e in the presence or absence of Ade3 and Ade4 at the FANCF and RNF2 sites. Values and error bars reflect the mean ± s.e.m. and n = 3 biologically independent experiments. All p values were calculated by two-sided t tests. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.