Figure 5.

Evaluation of CD80 expression in macrophages, in response to IVT-mRNA transfection with different chemistry

Staggered histogram of CD80 levels in macrophages transfected with low doses and high doses of IVT-mRNA with various cap modifications (A), as well as nucleotide modifications (C) along with untransfected, poly(I:C), the dsRNA positive control transfected in low dose, and activated macrophages. Activated cells were treated with LPS/IFN-γ. MFI of CD80 normalized to untransfected cells is indicated for macrophages transfected with low doses and high doses of mRNA with different cap modifications (B) and nucleotide modifications (D). Mock transfection refers to carrier (i.e., LipoMM) without mRNA. For each condition 125 ng∙mL⁻¹ and 500 ng∙mL⁻¹ of IVT-mRNA were used for transfection, referred here as low dose and high dose, respectively. Expression was measured 24 h after transfection. Values are presented as mean ± SD. Error bars indicate SD of three independent experiments from three individual donors (n = 3). ARCA, anti-reverse cap analog; MT, methyl-transferase; AnP, Antarctic phosphatase; Ψ, pseudouridine; me¹Ψ, N¹-Methylpseudouridine; 5moU, 5-methoxy-uridine; 5meC, 5-methyl-cytidine.