Figure 6.

Neat1 overexpression attenuates oxidative damage in mouse corneal endothelium during FECD pathogenesis

(A) Neat1-targeting CRISPR-dCas9 activation system. (B) ZO-1 staining showed the corneal endothelium morphology after Neat1 overexpression during FECD. Scale bars, 50 μm. sg-NC, sgRNA⁻ control; sg-Neat1, sgRNA-Neat1. (C) Coefficient of variation analysis from mouse corneal endothelium treated with sg-NC and sg-Neat1 rAAV (n = 3). (D) Comparison of OCT images from sg-NC, sg-Neat1, and NAC-treatment FECD mice. (E) Statistical analysis was used to analyze the effects of Neat1 and NAC treatment on CCT changes. n = 6 for each group. The red asterisk represents the difference between the sg-NC and NAC-treatment groups; the blue asterisk represents the difference between the sg-NC and sg-Neat1 groups; The black represents the difference between the sg-Neat1 and NAC groups. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, not significant (2-tailed t test).