Figure 1.

Step-by-step schematic representation of dissection and mounting

(A) Desired tissue is dissected and cleaned in 1× PBS.

(B) Fixation is done in 4% paraformaldehyde for 20 min and the tissue is washed 3 times for 10 min each before incubating overnight (∼8–16 h) in primary antibody. The tissue is then washed 3 times for 10 min each and incubated in secondary antibody for 2 h followed by washes. In this study we processed the tissue after secondary for TUNEL assay.

(C) The washed tissue was then mounted onto a slide and visualized under confocal microscope.