REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat-Elav-7E8A10 anti-elav (dilution 1:100)	Developmental Studies Hybridoma Bank	Antibody Registry ID: AB_528218
Goat anti-rat IgG conjugated with Cy5 (dilution 1:250)s	The Jackson Laboratory	Cat# 112-175-143
Chemicals, peptides, and recombinant proteins
Sodium Chloride	Sigma-Aldrich	Product# S7653
Potassium Chloride	Sigma-Aldrich	Product# P9333
Sodium Phosphate, Dibasic	Sigma-Aldrich	Product# S7907
Potassium Phosphate, Monobasic	Sigma-Aldrich	Product# P5655
Paraformaldehyde	Electron Microscopy Sciences	Cat# 15710
Vectashield	Vector labs	Cat# H1000
Triton X-100	Sigma-Aldrich	Cat# T8787
Normal Donkey Serum	Fisher scientific	Cat# 5058837
Sodium citrate	Thermo Fisher Scientific	Cat# S279
Critical commercial assays
TUNEL dilution buffer	Roche Diagnostics	Cat# 11966006001
In Situ Cell Death Detection Kit, TMR red	Roche Diagnostics	Cat# 12156792910
Experimental models: Organisms/strains
D. melanogaster Canton-S	Bloomington Drosophila Stock Center	BDSC: 64349; FlyBase: FBsn0000274
D. melanogaster ey-Gal4 (III)	(Hazelett et al., 1998)	N/A
D. melanogaster L2/CyO; ey-Gal4/ey-Gal4	(Singh et al., 2005, 2006; Singh and Choi, 2003)	N/A
D. melanogaster GMR-Gal4 w[∗]; P{w[+mC]=longGMR-Gal4}2	Bloomington Drosophila Stock Center	BDSC: 8605; FlyBase: FBti0058798
D. melanogaster GMR-hid, GMR-Gal4	A gift from Dr. Andreas Bergmann	N/A
D. melanogaster Viropana 4 (III)	(Mehta et al., 2021)	N/A
Software and algorithms
ImageJ software	National Institutes of Health	http://rsb.info.nih.gov/ij/
GraphPad Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/