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. 2022 Jan 28;3(1):101137. doi: 10.1016/j.xpro.2022.101137
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit antiperoxidase antibody linked to peroxidase (PAP1) Dako N/A
Chemicals, peptides, and recombinant proteins
Tris-HCl (pH 8.0) Thermo Fisher Scientific Cat#: 15568025
Sodium chloride (NaCl) Sigma-Aldrich Cat#: S3014
Glycerol Sigma-Aldrich Cat#: G5516
IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat#: I8896
Phenylmethanesulfonyl fluoride (PMSF) Sigma-Aldrich Cat#: P7626
Ethylenediaminetetraacetic acid solution (EDTA) Sigma-Aldrich Cat#: 03690
D,L-Dithiothreitol (DTT) Sigma-Aldrich Cat#: D0632
Magnesium acetate tetrahydrate Sigma-Aldrich Cat#: M5661
Imidazole Sigma-Aldrich Cat#: 68268
Calcium chloride dihydrate (CaCl2) Merck Cat#: 102382
2-Mercaptoethanol Sigma-Aldrich Cat#: M3148
Ethylene glycol-bis(2-aminoethyl ether)-N,N,N,N-tetra acetic acid (EGTA) Sigma-Aldrich Cat#: 03777
Sodium fluoride (NaF) Sigma-Aldrich Cat#: 201154
β-Glycerol phosphate disodium salt pentahydrate Sigma-Aldrich Cat#: 50020
Sodium pyrophosphate decahydrate Sigma-Aldrich Cat#: 221368
Sodium orthovanadate (Na3VO4) Sigma-Aldrich Cat#: 450243
cOmplete EDTA-free protease inhibitor cocktail Roche Cat#: COEDTAF-RO
PhosSTOP Sigma-Aldrich Cat#: 4906845001
IgG SepharoseTM 6 Fast Flow GE Healthcare Cat#. 17-0969-01
Calmodulin SepharoseTM 4B GE Healthcare Cat#. 17-0529-01
AcTEV protease Invitrogen Cat#: 12575023
Benzonase Nuclease Merck Cat#: 70664
Iodoacetamide Sigma-Aldrich Cat#: 1149
Trifluoroacetic acid Sigma-Aldrich Cat#: 80457
Acetonitrile Merck Cat#: 1000291000
Sequencing Grade Modified Trypsin Promega Cat#: V5111
LiChroprep RP-18 Merck Cat#: 1093030100
Experimental models: Organisms/strains
S. pombe: Strain background: h-ORF-TAP::KanMX6 N/A N/A
Other
Refrigerated Incubator with built-in Shaker N-BIOTEK Cat#: NB-250VQ
Refrigerated Large-volume Centrifuge N/A N/A
Large Capacity Cryogenic Grinder SPEX SamplePrep Cat#: 6875
Small Grinding Vial Set SPEX SamplePrep Cat#: 6751
Mid-Size Grinding Vial Set SPEX SamplePrep Cat#: 6881
Large Grinding Vial Set SPEX SamplePrep Cat#: 6801
MSE Soniprep 150 Ultrasonic Disintegrator MSE Cat#: JM458
Refrigerated High-speed Centrifuge Z 36 HK Hermle Cat#: 302.00 V03
Platform rotator IKA Cat#: 0004011000
Rotatory wheel IKA Cat#: 0004015000
Poly-Prep chromatography column (2 mL bed volume) Bio-Rad Cat#: 7311550
Econo-Pac chromatography column (20 mL bed volume) Bio-Rad Cat#: 7321010
Refrigerated Centrifuge Eppendorf Cat#: 5702 R
Refrigerated Microcentrifuge Eppendorf Cat#: 5418 R
Mini-PROTEAN Tetra Cell Bio-Rad Cat#: 1658000EDU
2-Gel Tetra and Blotting Module Bio-Rad Cat#: 1660827EDU
Protein LoBind Tube Eppendorf Cat#: 022431081
Vacuum Concentrator plus Eppendorf Cat#: 5305000304