| Reverse transcription thermocycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Pre-primer extension | 25°C | 10 min | 1 |
| DNA polymerization | 37°C | 120 min | 1 |
| Enzyme deactivation | 85°C | 5 min | 1 |
| Hold | 4°C | forever | |
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