Fig. 2.

Changes of markers of NET formation in anakinra pretreated and PMA-stimulated neutrophils. Cells were pre- treated with anakinra in concentrations of 100 ng/ml, 100 µg/ml and 1 mg/ml for 60, 30 and 10 minutes. Afterwards, cells were stimulated with PMA to induce NET formation. All data are values of absorbance/fluorescence normalized to PMA-stimulated controls. Normalized data for NE activity show significant results for pretreatment 60 and 30 min prior to PMA stimulation at different concentrations (A, B), while 10 min pretreatment was not significant for any con- centration (C). Normalized data for MPO are shown in (D-F) with no significant results for 60 min pretreatment with 100 µg/ml and 10 min pretreatment with 1 mg/ml. Results for cfDNA are shown in (G-I)