Figure 1.

Some digested dietary proteins decrease intestinal glucose absorption in vitro and ex vivo. (A) After 1-h apical pre-incubation with 5 mg/ml digested proteins, Caco-2/TC7 cells differentiated on transwells were apically exposed for 40 min to the same digested proteins supplemented with ¹⁴C-α-methyl-D-glucopyranoside (AMG). Glucose uptake was then quantified by measuring radioactivity in the cellular compartment. Results are expressed as % of control (blk SGID) – mean ± SD (**, p < 0.01; ***, p < 0.001; and ****, p < 0.0001 compared to control – one-way ANOVA followed by Dunnett's test). (B) Rat jejunal sacs were filled with 31.25 mg/mL digested proteins (hemoglobin, ovalbumin, casein, pea proteins, fish gelatin, and gluten) and ³H-glucose. Glucose transport was quantified by measuring radioactivity in the incubation medium for 20 min and AUC was then calculated. Results are expressed as % of control (blk SGID) – mean ± SD (**, p < 0.01 compared to control – one-way ANOVA followed by Dunnett's test).