| Antibodies |
| Rabbit polyclonal anti-mCatSper1 |
Laboratory of David E. Clapham; Ren et al., 2001
|
Cat#CatSper1; RRID:AB_2314097 |
| Rabbit polyclonal anti-mCatSper2 |
Laboratory of David L. Garbers; Quill et al., 2001
|
N/A |
| Rabbit polyclonal anti-mCatSper3 |
Laboratory of David E. Clapham; Qi et al., 2007
|
N/A |
| Rabbit polyclonal anti-mCatSper4 |
Laboratory of David E. Clapham; Qi et al., 2007
|
N/A |
| Rabbit polyclonal anti-mCatSperβ |
Laboratory of David E. Clapham; Chung et al., 2011
|
N/A |
| Rabbit polyclonal anti-mCatSperδ |
Laboratory of David E. Clapham; Chung et al., 2011
|
N/A |
| Rabbit polyclonal anti-mCatSperζ |
Laboratory of Jean-Ju Chung; Chung et al., 2017
|
N/A |
| Rabbit polyclonal anti-hCatSperζ |
Laboratory of Jean-Ju Chung; Chung et al., 2017
|
N/A |
| Rabbit polyclonal anti-mEFCAB9 |
This study |
N/A |
| Rabbit polyclonal anti-GFP (FL) |
SantaCruz |
Cat# sc-8334; RRID:AB_641123 |
| Mouse monoclonal anti-PMCA4 (clone JA9) |
Novus Biologicals |
Cat# NB300-569; RRID: AB_2061726 |
| Mouse monoclonal anti-Flag (clone M2) |
Sigma-Aldrich |
Cat# F3165; RRID:AB_259529 |
| Mouse monoclonal anti-acetylated tubulin |
Sigma-Aldrich |
Cat# 7451; RRID:AB_609894 |
| Mouse monoclonal anti-Calmodulin |
Upstate |
Cat# 05-173; RRID:AB_309644 |
| Mouse monoclonal anti-phosphotyrosine (clone 4G10) |
EMD Millipore |
Cat# 05-321; RRID:AB_309678 |
| Mouse monoclonal anti-HA (clone 2–2.2.14) |
Pierce |
Cat# 26183; RRID:AB_2533056 |
| Mouse monoclonal anti-MYC (clone 9E10) |
SantaCruz |
Cat# sc-40; RRID:AB_627268 |
| Mouse monoclonal anti-HA (clone 6E2), HRP-conjugated |
CST |
Cat# 2999S; RRID:AB_1264166 |
| Mouse monoclonal anti-MYC (clone 9B11), HRP-conjugated |
CST |
Cat# 2040S; RRID:AB_10707162 |
| Mouse monoclonal anti-V5 (clone E10/V4RR), HRP-conjugated |
Invitrogen |
Cat# MA5-15253-HRP; RRID:AB_2537645 |
| Goat anti-mouse IgG-HRP |
Jackson ImmunoResearch |
Cat# 115-035-003; RRID:AB_10015289 |
| Goat anti-rabbit IgG-HRP |
Jackson ImmunoResearch |
Cat# 111-035-144; RRID:AB_2307391 |
| Rat anti-mouse IgG Trueblot (clone eB144) |
Rockland |
Cat# 18-8817-31; RRID:AB_2610850 |
| Anti-V5 Agarose Affinity Gel (clone V5-10) |
Sigma Aldrich |
Cat# A7345 |
| Anti-HA Magnetic Beads (clone 2–2.2.14) |
Pierce |
Cat# 88836 |
| Bacterial and Virus Strains |
| NEB 10-beta Electrocompetent E. coli (High Efficiency) |
NEB |
Cat# C3019H |
| BL21-CodonPlus (DE3)-RIL competent cells |
Agilent Technology |
Cat# 230245 |
| Chemicals, Peptides, and Recombinant Proteins |
| EmbryoMax M2 Medium (1X), Liquid, with phenol red |
EMD Millipore |
Cat# MR-015-D |
| EmbryoMax Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF |
EMD Millipore |
Cat# MR-070-D |
| Dulbecco’s Modified Eagle Medium (DMEM) |
GIBCO |
Cat# 12100-046 |
| HAM’s F12 |
GIBCO |
Cat# 21700-075 |
| FBS |
Thermofisher |
Cat# 10437-028 |
| MG-132 (CAS 133407-82-6) |
Calbiochem |
Cat# 474790 |
| Isopropyl β-D-thiogalactopyranoside (IPTG) |
AmericanBio |
Cat# AB00841 |
| BAPTA-AM (CAS 126150-97-8) |
Calbiochem |
Cat# 196419 |
| Pluronic F-127 |
Invitrogen |
Cat# P3000MP |
| Dimethyl Sulfoxide, DMSO |
AmericanBio |
Cat# AB03091 |
| Critical Commercial Assays |
| Multiple Tissue Panels, cDNA (Mouse MTCTM Panel I) |
Clontech |
Cat# 636745 |
| Deposited Data |
| Raw images of immunoblot |
This study |
https://doi.org/10.17632/mv52ykks6x.1
|
| BLASTP, NCBI Database |
National Center for Biotechnology Information |
https://blast.ncbi.nlm.nih.gov/Blast.cgi
|
| BLASTP, JGI Database |
Joint Genome Institute |
https://genome.jgi.doe.gov
|
| Mouse ENCODE transcriptome data |
Yue et al., 2014
|
BioProject: PRJNA66167 |
| Experimental Models: Cell Lines |
| Human Embryonic Kidney (HEK) 293T |
ATTC |
Cat# CRL-3216; RRID:CVCL_0063 |
| Human Embryonic Kidney (HEK) human CatSper1-GFP stable expression cell |
Laboratory of David E. Clapham |
N/A |
| Experimental Models: Organisms/Strains |
| Mouse: B6.129S4-Catsper1tm1Clph/J
|
Laboratory of David E. Clapham; Ren et al., 2001
|
RRID:IMSR_JAX:018311 |
| Mouse: B6.129S4-Catsperdtm1.1Clph/J
|
Laboratory of David E. Clapham; Chung et al., 2011
|
RRID:IMSR_JAX:021451 |
| Mouse: Catsperztm1Clph (from ES cell line 1700019N12Riktm1(KOMP)Mbp) |
Laboratory of David E. Clapham; Chung et al., 2017
|
N/A |
| Mouse:Efcab9tm1CHNG
|
This study |
N/A |
| Mouse:C57BL/6NCrl |
Charles River Laboratories |
Crl:029 |
| Mouse:B6D2F1/Crl |
Charles River Laboratories |
Crl:099 |
| Recombinant DNA |
| Mammalian expression vector for mouse EFCAB9-HA (phCMV3-mEfcab9) |
This study |
N/A |
| Mammalian expression vector for mouse CatSperz-V5 (pCAG-mCatSperz-V5) |
Laboratory of David E. Clapham |
N/A |
| Mammalian bi-cystronical expression vector for mouse CatSperz-V5 and mouse EFCAB9-HA (phCMV3-mCatSperz-V5-P2A-mEfcab9) |
This study |
N/A |
| Mammalian expression vector for mouse CatSper1-Flag (phCMV3-mCatSper1-Flag) |
This study |
N/A |
| Mammalian expression vector for mouse CatSper2-Flag (phCMV3-mCatSper2-Flag) |
This study |
N/A |
| Mammalian expression vector for mouse CatSper3-Flag (phCMV3-mCatSper3-Flag) |
This study |
N/A |
| Mammalian expression vector for mouse CatSper4-Flag (phCMV3-mCatSper4-Flag) |
This study |
N/A |
| Mammalian expression vector for mouse Flag-CatSperb (pTracer2-Flag-mCaSperb) |
Laboratory of David E. Clapham |
N/A |
| Mammalian expression vector for mouse CatSperd (pcDNA3-mCatSperd) |
Laboratory of David E. Clapham; Chung et al., 2011
|
N/A |
| Mammalian expression vector for mouse CatSperd-V5 (pCAG-mCatSperd-V5) |
Laboratory of David E. Clapham; Chung et al., 2011
|
N/A |
| Mammalian expression vector for human EFCAB9-HA (phCMV3-hEFCAB9-HA) |
This study |
N/A |
| Mammalian expression vector for human CatSperz-V5 (phCMV3-hCatSpere-V5) |
This study |
N/A |
| Mammalian expression vector for Flag-human CatSpere (phCMV3-Flag-hCatSpere) |
This study |
N/A |
| Mammalian expression vector for Flag-human CatSperg (phCMV3-Flag-hCatSperg) |
This study |
N/A |
| Mammalian bi-cystronical expression vector for human EFCAB9-HA amd human CatSperz-MYC (pcDNA3.1(-)-hEfcab9-HA-P2A-hCatSperz-MYC) |
This study |
N/A |
| Bactrerial expression vector for GST-mouse EFCAB9 (pGEX-6P2-mEfcab9) |
This study |
N/A |
| Bactrerial expression vector for GST-mouse EFCAB9 (pET43a-mEfcab9) |
This study |
N/A |
| Bactrerial expression vector for 6xHis-pET32a-gb1-mCatSperzEFCAB9 (pET32a-gb1-CatSperz) |
This study |
N/A |
| Bactrerial expression vector for GST-mouse EFCAB92Mut (D72N/E160Q), (pGEX-6P2-mEfcab92Mut
|
This study |
N/A |
| Bactrerial expression vector for GST-mouse EFCAB94Mut (D72N/D82N and D149N/E160Q), (pGEX-6P2-mEfcab94Mut) |
This study |
N/A |
| Bacterial expression vector for GST-mouse CatSper1 N terminus (1–150) (pGex-2T-mCatSper1-N150) |
Laboratory of David E. Clapham |
N/A |
| Bacterial expression vector for mouse 6xHis-CatSper1 C terminus (574–686)-Flag (pET32a-mCatSper1-C-Flag) |
This study |
N/A |
| Mouse Efcab9 ORF clone 6771929 |
Dharmacon |
Cat# MMM1013-202843024 |
| Human Efcab9 ORF clone OHu00121D |
GenScript |
Cat# OHu00121D |
| Human CatSpere ORF clone 5269307 |
Dharmacon |
Cat# MHS6278-202808312 |
| Human CatSpere ORF clone 4823002 |
Dharmacon |
Cat# MHS1010-202742776 |
| pGEX-6P2 |
GE Healthcare |
Cat# 28954650 |
| pET43.1a(+) |
Novagen |
Cat# 70939 |
| pET32a(+) |
Novagen |
Cat# 69015 |
| Software and Algorithms |
| Fiji |
Schindelin et al., 2012
|
http://fiji.sc
|
| Zen 2012 SP2 |
Carl Zeiss |
https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html
|
| SCA evolution |
Microptic |
https://www.micropticsl.com
|
| R |
R Foundation |
https://www.r-project.org/
|
| MEGA6 |
Tamura et al., 2013
|
https://www.megasoftware.net/
|
| Other |
| Glutathione agarose |
Pierce |
Cat# 16100 |
| HisPur Ni-NTA Resin |
Pierce |
Cat# 88221 |
| Protein A/G PLUS-Agarose |
SantaCruz |
Cat# sc-2003 |
| SureBeads Protein A Magnetic Beads |
BioRad |
Cat# 1614011 |
| SureBeads Protein G Magnetic Beads |
BioRad |
Cat# 1614021 |
| CellVision 4 Chamber 20 micron |
CellVision |
Cat# CV 1020-4CH |
