. Author manuscript; available in PMC: 2022 Feb 2.

Published in final edited form as: Methods Mol Biol. 2021;2304:111–130. doi: 10.1007/978-1-0716-1402-0_5

Table 1.

Common configurations for Airyscan SR imaging. Select these components in the “Imaging Setup” window. The options for each filter set are listed in Fig. 2.

Configuration	Tracks	Laser	Main Beam Splitter (MBS)	Second Beam Splitter (SBS	Emission Dual Filters	Detector
4 Color Line Switch	1	633	MBS 488/561/633; MBS 405	Option #1		Ch A
	2	561		Plate	Plate
	3	488		Option #2
	4	405		SBS BP 420-460 +LP 500	BP495-550+ LP570
4 Color Frame Switch	1	633	MBS 488/561/633; MBS 405	SBS LP 660	BP 570-620 + LP645	Ch A
	2	561		SBS SP 615	BP 570-620 + LP645
	3	488		SBS BP 420-460 +LP 500	BP420-480 + BP495-550
	4	405		Plate	BP420-480 + BP495-550
2 Color (RG) Line Switch	1	561	MBS 488/561	SBS SP 615	BP 495-550 + LP 570	Ch A
2 Color (RG) Line Switch	2	488	MBS 488/561	SBS SP 615	BP 495-550 + LP 570
2 Color (RG) Frame Switch	1	561	MBS 488/561	SBS SP 615	BP 570-620 + LP645	Ch A
2 Color (RG) Frame Switch	2	488	MBS 488/561	SBS SP 615	BP420-480 + BP495-550	Ch A
3 Color (RGB)+Transmitted Light Frame Switch	1	561	MBS 488/561/633; MBS 405	SBS SP 615	BP 570-620 + LP645	Ch A
	2	488		Plate	BP420-480 + BP495-550
	3	405		Plate	BP420-480 + BP495-550
	4	633		not affect	not affect	T-PMT